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for reference- all the water you need is to cover your fish and media, the bacteria dies because of lack of oxygen (aerobic and anaerobic), not because of the ammonia or anything, as long as your water is agitated it should be fine. I drove 1500 kms with my tank busters and massive tank, arowanas, gars, tons of fish, they were in bags for 30 hours and everyone came out fine. other than that just imagine giving your fish a massive 70% water change! hardly any bacteria live in the water, its all in your gravel/sand, and media.
 
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